Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-18
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
Page 17-25
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
Page F-2
... fragment of E. coli DNA polymerase I GAATTOH 3 ' CTTAAр5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : dATP dTTP dCTP A OH 3 3 ' dGTP TTCGAр5 ' Klenow fragment of E. coli DNA polymerase ...
... fragment of E. coli DNA polymerase I GAATTOH 3 ' CTTAAр5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : dATP dTTP dCTP A OH 3 3 ' dGTP TTCGAр5 ' Klenow fragment of E. coli DNA polymerase ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml