Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii 6.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii 6.
Page B-23
... Electrophoresis Buffers Working solution Buffer Tris - acetate ( TAE ) 1 × : 0.04 M Tris - acetate 0.001 M EDTA Tris ... gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost ...
... Electrophoresis Buffers Working solution Buffer Tris - acetate ( TAE ) 1 × : 0.04 M Tris - acetate 0.001 M EDTA Tris ... gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost ...
Page 1-14
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml