Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 89
Page 16-31
... method is simple and appears to be efficient , it is comparatively new and untested ( but see Felgner and Holm 1989 ; Maurer 1989 ) . • Direct microinjection into nuclei ( Capecchi 1980 ) . Although this method has the advantage of not ...
... method is simple and appears to be efficient , it is comparatively new and untested ( but see Felgner and Holm 1989 ; Maurer 1989 ) . • Direct microinjection into nuclei ( Capecchi 1980 ) . Although this method has the advantage of not ...
Page 1-6
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
Page 1-7
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml