Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 54
Page xviii
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 18-21
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
Page 18-26
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml