Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-42
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
Page 9-56
... Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they are perfectly matched . Duplexes with a single base - pair mismatch are significantly less stable and dissociate at a lower temperature than ...
... Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they are perfectly matched . Duplexes with a single base - pair mismatch are significantly less stable and dissociate at a lower temperature than ...
Page 15-72
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector