Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page xii
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
Page 10-54
... DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , ...
... DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , ...
Page 13-8
... polymerase I is the enzyme of choice for determining the sequence of tracts of DNA that lie within 250 bases of the 5 ' terminus of the primer . It is not recommended for sequencing longer segments ... DNA 13.8 DNA Sequencing A Diluent A 8.
... polymerase I is the enzyme of choice for determining the sequence of tracts of DNA that lie within 250 bases of the 5 ' terminus of the primer . It is not recommended for sequencing longer segments ... DNA 13.8 DNA Sequencing A Diluent A 8.
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector