Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 64
Page 8-50
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
Page 12-14
... antibody bound to its antigen on nitrocellulose filters are : 125 I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus ...
... antibody bound to its antigen on nitrocellulose filters are : 125 I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus ...
Page 12-20
... antibody - antibody - AP complexes are located using the sub- strate 5 - bromo - 4 - chloro - 3 - indolyl phosphate ( BCIP ) in combination with nitro blue tetrazolium ( NBT ) , which detects the precipitated indoxyl group . Stock ...
... antibody - antibody - AP complexes are located using the sub- strate 5 - bromo - 4 - chloro - 3 - indolyl phosphate ( BCIP ) in combination with nitro blue tetrazolium ( NBT ) , which detects the precipitated indoxyl group . Stock ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector