Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 43
Page 10-7
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at -20 ° C . 10x Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mm dithiothreitol 500 μg / ml ...
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) Divide the stock solution into small aliquots and store them at -20 ° C . 10x Ligation buffer 0.5 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 100 mm dithiothreitol 500 μg / ml ...
Page 12-34
... dithiothreitol to a final concentration of 1 mM . 10x Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 40 mM KCI 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
... dithiothreitol to a final concentration of 1 mM . 10x Binding buffer 250 mM HEPES ( pH 7.9 ) 30 mM MgCl2 40 mM KCI 5. Decant the second batch of denaturation solution into a graduated cylin- der . Dilute the solution with an equal ...
Page 15-64
... dithiothreitol m Heat the mixture for 5 minutes to 20 ° C above the theoretical T. of a perfect hybrid formed by the mutagenic oligonucleotide , calculated from the formula Tm = 4 ( G + C ) + 2 ( A + T ) . Transfer the tube containing ...
... dithiothreitol m Heat the mixture for 5 minutes to 20 ° C above the theoretical T. of a perfect hybrid formed by the mutagenic oligonucleotide , calculated from the formula Tm = 4 ( G + C ) + 2 ( A + T ) . Transfer the tube containing ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector