Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-16
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
Page 10-27
... DOUBLE - STRANDED DNA TEMPLATES BY BACTERIOPHAGE DNA- DEPENDENT RNA POLYMERASES The technical advance that made it possible to synthesize single - stranded RNA probes of high specific activity was the development of plasmid vectors ...
... DOUBLE - STRANDED DNA TEMPLATES BY BACTERIOPHAGE DNA- DEPENDENT RNA POLYMERASES The technical advance that made it possible to synthesize single - stranded RNA probes of high specific activity was the development of plasmid vectors ...
Page 14-28
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector