Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-44
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
... infected bacteria or induced lysogens , essentially as described for Agt11 . In addition , AZAP fusion proteins may be expressed from high - copy - number plasmids following the excision of the DNA insert from the bacteriophage vector ...
Page 8-79
... infect a restriction- competent strain of E. coli . During amplification , it is important to suppress the production ... infected culture on a 150 - mm petri dish containing NZCYM agar . Incubate for 12 hours at 42 ° C ( to prevent the ...
... infect a restriction- competent strain of E. coli . During amplification , it is important to suppress the production ... infected culture on a 150 - mm petri dish containing NZCYM agar . Incubate for 12 hours at 42 ° C ( to prevent the ...
Page 12-16
... infected . In each tube , mix 0.1 ml of the plating bacteria with 0.1 ml of SM containing 3 × 10 * pfu ( 90 - mm plates ) or 105 pfu ( 150 - mm plates ) of the bacteriophage A expression library . Incubate the infected bacteria for 20 ...
... infected . In each tube , mix 0.1 ml of the plating bacteria with 0.1 ml of SM containing 3 × 10 * pfu ( 90 - mm plates ) or 105 pfu ( 150 - mm plates ) of the bacteriophage A expression library . Incubate the infected bacteria for 20 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector