Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 12-32
... ligase ( 1 Weiss unit / μl ) and 1 μl of 50 mм ATP . Incubate the mixture for 12 hours at 16 ° C . At least three different assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New ...
... ligase ( 1 Weiss unit / μl ) and 1 μl of 50 mм ATP . Incubate the mixture for 12 hours at 16 ° C . At least three different assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New ...
Page 13-24
... ligase buffer 5 mM гATP 30 % w / v polyethylene glycol ( PEG 8000 ) ( optional - see note to step 1 ) H2O to 25 μl bacteriophage T4 DNA ligase for cohesive termini for blunt termini 2.5 με 2.5 με 5.0 με 1.0 Weiss unit 5.0 Weiss units ...
... ligase buffer 5 mM гATP 30 % w / v polyethylene glycol ( PEG 8000 ) ( optional - see note to step 1 ) H2O to 25 μl bacteriophage T4 DNA ligase for cohesive termini for blunt termini 2.5 με 2.5 με 5.0 με 1.0 Weiss unit 5.0 Weiss units ...
Page 15-30
... ligase therefore will ligate 50 % of the HindIII fragments of bacteriophage A ( 5 μg ) in 30 minutes at 16 ° C . Throughout this manual , bac- teriophage T4 DNA ligase is given in Weiss units . 14. Heat the ligation reaction to 68 ° C ...
... ligase therefore will ligate 50 % of the HindIII fragments of bacteriophage A ( 5 μg ) in 30 minutes at 16 ° C . Throughout this manual , bac- teriophage T4 DNA ligase is given in Weiss units . 14. Heat the ligation reaction to 68 ° C ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector