Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-38
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
Page 8-57
... lysogenic for a bacteriophage λ that carries the gene ( polA ) that codes for the holoenzyme of E. coli DNA polymerase I ( Murray and Kelley 1979 ) . Most commercial suppliers sell this enzyme in buffer containing 50 % glycerol ...
... lysogenic for a bacteriophage λ that carries the gene ( polA ) that codes for the holoenzyme of E. coli DNA polymerase I ( Murray and Kelley 1979 ) . Most commercial suppliers sell this enzyme in buffer containing 50 % glycerol ...
Page 9-12
... lysogenic for bacteriophage P2 . Nonrecombinants formed by re - ligation of the central stuffer fragment into the arms are unable to grow efficiently on strains of E. coli that are lysogenic for bacteriophage P2 . • If the bacteriophage ...
... lysogenic for bacteriophage P2 . Nonrecombinants formed by re - ligation of the central stuffer fragment into the arms are unable to grow efficiently on strains of E. coli that are lysogenic for bacteriophage P2 . • If the bacteriophage ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector