Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-16
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable ...
Page 14-28
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Protocol II : Sequencing Single - stranded DNA Templates Generated by Asymmetric Amplification In this method ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Protocol II : Sequencing Single - stranded DNA Templates Generated by Asymmetric Amplification In this method ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
Page 15-53
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Target DNA All oligonucleotide - mediated mutagenesis procedures require a target DNA that is at least partially single - stranded . This can be prepared ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Target DNA All oligonucleotide - mediated mutagenesis procedures require a target DNA that is at least partially single - stranded . This can be prepared ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector