Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 85
Page 11-50
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
... solution of TMACI or TEACI through a Whatman No. 1 paper . 4. Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 - micron pore size ) . Store the filtered solution in dark bottles at room temperature . 5. Measure ...
Page 13-52
... solution in a small Erlen- meyer flask . b . Place 35 ml of 6 % acrylamide / urea top solution in a 100 - ml beaker . c . Add 40 μl of 10 % ammonium persulfate to the 6 % acrylamide / urea bottom solution . Mix the solution by rapid ...
... solution in a small Erlen- meyer flask . b . Place 35 ml of 6 % acrylamide / urea top solution in a 100 - ml beaker . c . Add 40 μl of 10 % ammonium persulfate to the 6 % acrylamide / urea bottom solution . Mix the solution by rapid ...
Page 13-59
... solutions of dNTPs and ddNTPs . We recommend making up three sets of working solutions : • A chase solution that contains all four dNTPs , each at a concentration of 0.5 mm . Dispense the chase solution into aliquots and store them at ...
... solutions of dNTPs and ddNTPs . We recommend making up three sets of working solutions : • A chase solution that contains all four dNTPs , each at a concentration of 0.5 mm . Dispense the chase solution into aliquots and store them at ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector