Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 58
Page 8-36
... stored at -70 ° C ; more frequently , they were maintained on the surfaces of nitrocellulose filters ( Hanahan and Meselson 1983 ) . In either case , the results were unsatisfactory . The libraries were difficult to preserve without ...
... stored at -70 ° C ; more frequently , they were maintained on the surfaces of nitrocellulose filters ( Hanahan and Meselson 1983 ) . In either case , the results were unsatisfactory . The libraries were difficult to preserve without ...
Page 9-33
... stored at 4 ° C , it should be heated to 56 ° C for 2-3 minutes before it is applied to the gel . This disrupts any base pairing that may have occurred between protruding cohesive termini . The gel should be run slowly ( < 1 V / cm ) to ...
... stored at 4 ° C , it should be heated to 56 ° C for 2-3 minutes before it is applied to the gel . This disrupts any base pairing that may have occurred between protruding cohesive termini . The gel should be run slowly ( < 1 V / cm ) to ...
Page 9-49
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate pmoles polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled radiolabeled DNA recombinant remove restriction enzyme room temperature sample screening sequencing reactions single-stranded DNA solution specific activity stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH unlabeled vector