Molecular Cloning: A Laboratory Manual, Volume 2Cold Spring Harbor Laboratory Press, 2001 |
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Acad agarose gel aliquots amplification reaction amplified DNA annealing antibody Appendix bacteriophage M13 bacteriophage T4 Biol BioTechniques catalyzed cDNA clones cDNA libraries cells centrifuge Chapter cloning concentration containing cycle ddNTP denaturation detection digestion DNA fragments DNA sequencing DNA templates dNTPs double-stranded DNA efficiency encoding ethanol exonuclease expression filters gel electrophoresis gene genomic DNA H₂O hybridization information panel Klenow fragment labeled length ligation method microfuge tube minutes molecules mRNA mutagenesis mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligonucleotide oligonucleotide primers PCR products plaques plasmid plasmid DNA polyacrylamide gel polymerase chain reaction polymerization preparation probes Proc protein purified radioactivity radiolabeled reaction mixture reagents listed recombinant requires the reagents residues restriction enzyme reverse transcriptase RNase RNase H room temperature samples screening sequencing reactions single-stranded DNA Step strand target DNA target sequence template DNA termini thermal cycler thermostable DNA polymerase tion vector vitro µg/ml