Molecular Cloning: Ch. 15. Expression of cloned genes in Escherichia coli |
Contents
Chapter | 15-3 |
Expression of Cloned Genes in Escherichia coli 15 | 15-12 |
INFORMATION PANELS | 15-55 |
Copyright | |
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Common terms and phrases
Acad activity aliquots amino acids analysis anti-TSH antibody assay autoclaving B-galactosidase bacterial baculovirus bait BIAcore binding Biochem Biol buffer cDNA cell lines centrifugation Chapter cleavage cloning codon complex concentration containing culture detection Dilute DNA polymerase DNase domain double-stranded DNA EDTA efficiency electrophoresis electroporation encoded epitope Escherichia coli exonuclease filter fluorescent protein fragments fusion protein gene expression genome green fluorescent protein H₂O hybrid Incubate interactions kinase lacZ ligand luciferase mammalian cells medium membrane method mg/ml Mg2+ microfuge minutes molecular molecules mutations NaCl Natl nuclear Nucleic Acids oligonucleotides peptide phage phosphate plasmid plates Prepare probe Proc promoter protease protein-protein Protocol purification reaction reagents recombinant restriction enzymes RNase room temperature sample screening selection sequence single-stranded specific ẞ-galactosidase Step sterile stock solutions Store strain substrates surface synthesis tein termini tetracycline tion transcription transfection transfer transformation Tris-Cl pH tube vectors X-gal yeast µg/ml