Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 33
... Base Pairing . ( a ) Structural formulas for thy- mine : adenine ( TA ) and cytosine : guanine ( CG base pairs ) . The bases are flat structures composed of hydrogen , carbon , nitrogen , and oxygen atoms . The solid lines represent ...
... Base Pairing . ( a ) Structural formulas for thy- mine : adenine ( TA ) and cytosine : guanine ( CG base pairs ) . The bases are flat structures composed of hydrogen , carbon , nitrogen , and oxygen atoms . The solid lines represent ...
Page 34
A Guide for the Curious Karl Drlica. units we have now defined as nucleotide pairs , or base pairs , corre- spond to the frames in a motion picture film . That is , the " genetic let- ters " mentioned earlier represent the chemical base ...
A Guide for the Curious Karl Drlica. units we have now defined as nucleotide pairs , or base pairs , corre- spond to the frames in a motion picture film . That is , the " genetic let- ters " mentioned earlier represent the chemical base ...
Page 107
... base pairs remain between cut sites . Under most conditions , four base pairs are not enough to hold DNA together , so the DNA molecule separates into fragments ( Figure 7-1 ) . Many restriction endonucleases are commercially available ...
... base pairs remain between cut sites . Under most conditions , four base pairs are not enough to hold DNA together , so the DNA molecule separates into fragments ( Figure 7-1 ) . Many restriction endonucleases are commercially available ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease restriction map retroviruses reverse transcriptase ribosome ribozyme Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses