Cytopathology of the UterusCovers squamous intraepithelial lesions lesions of the endocervix technical considerations etc. |
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Page 425
We describe the procedure used in our laboratory to demonstrate glycogen in
specimens of the uterine cervix . 20.11A Tissue Preparation Prepared Reagents (
see Procedure 20.7 ) 2.5 % Glutaraldehyde solution Cacodylate buffer 1 % OsO4
...
We describe the procedure used in our laboratory to demonstrate glycogen in
specimens of the uterine cervix . 20.11A Tissue Preparation Prepared Reagents (
see Procedure 20.7 ) 2.5 % Glutaraldehyde solution Cacodylate buffer 1 % OsO4
...
Page 431
Alexander Meisels, Carol Morin. 20.12 In Situ Hybridization Technique 20.12A
General Preparations and Equipment Carnoy's fixative ( see Procedure 20.1.1 )
Neutral buffered formalin ( see Procedure 20.1.3 ) Absolute ethanol Xylene
Double ...
Alexander Meisels, Carol Morin. 20.12 In Situ Hybridization Technique 20.12A
General Preparations and Equipment Carnoy's fixative ( see Procedure 20.1.1 )
Neutral buffered formalin ( see Procedure 20.1.3 ) Absolute ethanol Xylene
Double ...
Page 438
Add 0.58 g NaCl to the tube and mix by inversion . d . Incubate at 42 ° C for 15 to
20 minutes . Procedure 1. Place each membrane in a plastic bag . 2. Pour 10 mL
prehybridization mixture into the bag . 3. Squeeze out air bubbles and seal . 4.
Add 0.58 g NaCl to the tube and mix by inversion . d . Incubate at 42 ° C for 15 to
20 minutes . Procedure 1. Place each membrane in a plastic bag . 2. Pour 10 mL
prehybridization mixture into the bag . 3. Squeeze out air bubbles and seal . 4.
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