Cytopathology of the Uterus |
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Page 420
... buffer for 60 minutes each . Note : Tissues can remain for many days or weeks in this cold buffer before going to step 4 . 4. Fix in cacodylate - buffered 1 % OsO , for 90 minutes at 4 ° C . 4 5. Rinse three times with 0.1 mol / L ...
... buffer for 60 minutes each . Note : Tissues can remain for many days or weeks in this cold buffer before going to step 4 . 4. Fix in cacodylate - buffered 1 % OsO , for 90 minutes at 4 ° C . 4 5. Rinse three times with 0.1 mol / L ...
Page 425
... buffer for 1 hour . 3. Wash in 0.1 mol / L cacodylate buffer for 1 hour . 4. Wash in 0.1 mol / L cacodylate buffer for 1 hour . 5. Fix in 1 % OsO for 90 minutes . 4 6. Wash in 0.1 mol / L cacodylate buffer for 10 minutes . 7. Wash in ...
... buffer for 1 hour . 3. Wash in 0.1 mol / L cacodylate buffer for 1 hour . 4. Wash in 0.1 mol / L cacodylate buffer for 1 hour . 5. Fix in 1 % OsO for 90 minutes . 4 6. Wash in 0.1 mol / L cacodylate buffer for 10 minutes . 7. Wash in ...
Page 449
... buffer . Biopsy specimens are finely sliced with scissors and placed in 2.25 mL extraction buffer . 2. Add 250 μL proteinase K ( 5 mg / mL ) ( incubated 1 hour at 37 ° C ) . 3. Incubate at 37 ° C until digestion is completed . 4. Add 25 ...
... buffer . Biopsy specimens are finely sliced with scissors and placed in 2.25 mL extraction buffer . 2. Add 250 μL proteinase K ( 5 mg / mL ) ( incubated 1 hour at 37 ° C ) . 3. Incubate at 37 ° C until digestion is completed . 4. Add 25 ...
Contents
Hormonal Cytology | 6 |
J Ernest Ayre | 12 |
The Procurement of Cytologic Specimens | 19 |
Copyright | |
33 other sections not shown
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abnormal acid Acta Cytol adenocarcinoma antibody antigen ASCUS atypia benign Bethesda System biopsy cell carcinoma cellular cervical cancer cervical carcinoma cervical intraepithelial neoplasia cervical smears chromatin Clin clinical colposcopic condyloma contain cytology cytopathology cytoplasm cytotechnologists detection diagnosis distilled water double-distilled water dysplasia Electron endocervical endometrial cancer endometrium Epidemiology epithelial estrogen ethanol Figure gene genital warts genome glandular cells HGSIL high-grade squamous intraepithelial histologic hormonal HPV infection HPV types HPV-DNA human papillo human papillomavirus human papillomavirus infection human papillomavirus type hybridization Image invasive keratin koilocytes laboratory low power malignant mavirus medium power Meisels membrane microscope minutes mol/L morphologic nuclear nuclei Obstet Gynecol Papanicolaou smears papillomavirus DNA Pathol pathologists patients polymerase chain reaction primers probes Procedure protein Reagents rescreening risk sample screening sequences sexual slides solution specific specimens squamous intraepithelial lesion staining technique tion Tissue section tumor uterine cervix viral Virol virus women