Cytopathology of the Uterus |
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Page 428
... concentration of salt is raised , or if the pH is lowered , complementary single strands reassociate or anneal to form stable duplexes or hybrids . This experimental reassociation is termed molec- ular hybridization ( Figure 20.2 ) ...
... concentration of salt is raised , or if the pH is lowered , complementary single strands reassociate or anneal to form stable duplexes or hybrids . This experimental reassociation is termed molec- ular hybridization ( Figure 20.2 ) ...
Page 438
... concentration of probes must be made of 50 % of each . 3. Reseal the plastic bag . 4. Incubate overnight with a constant agitation at 42 ° C . 20.13F Washing Protocol Prepared Reagents 1. 20x SSC ( see Procedure 20.12A.9 ) 2. 10 % SDS ...
... concentration of probes must be made of 50 % of each . 3. Reseal the plastic bag . 4. Incubate overnight with a constant agitation at 42 ° C . 20.13F Washing Protocol Prepared Reagents 1. 20x SSC ( see Procedure 20.12A.9 ) 2. 10 % SDS ...
Page 449
... concentration of DNA by spectrophotometry . 49,86 25. Precipitate DNA by adding 10 μL 5 mol / L NaCl and 2 mL anhydrous ethanol and leaving tubes at −20 ° C overnight . 26. Centrifuge precipitated DNA at 9000 rpm for 45 minutes . 27 ...
... concentration of DNA by spectrophotometry . 49,86 25. Precipitate DNA by adding 10 μL 5 mol / L NaCl and 2 mL anhydrous ethanol and leaving tubes at −20 ° C overnight . 26. Centrifuge precipitated DNA at 9000 rpm for 45 minutes . 27 ...
Contents
Hormonal Cytology | 6 |
J Ernest Ayre | 12 |
The Procurement of Cytologic Specimens | 19 |
Copyright | |
33 other sections not shown
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abnormal acid Acta Cytol adenocarcinoma antibody antigen ASCUS atypia benign Bethesda System biopsy cell carcinoma cellular cervical cancer cervical carcinoma cervical intraepithelial neoplasia cervical smears chromatin Clin clinical colposcopic condyloma contain cytology cytopathology cytoplasm cytotechnologists detection diagnosis distilled water double-distilled water dysplasia Electron endocervical endometrial cancer endometrium Epidemiology epithelial estrogen ethanol Figure gene genital warts genome glandular cells HGSIL high-grade squamous intraepithelial histologic hormonal HPV infection HPV types HPV-DNA human papillo human papillomavirus human papillomavirus infection human papillomavirus type hybridization Image invasive keratin koilocytes laboratory low power malignant mavirus medium power Meisels membrane microscope minutes mol/L morphologic nuclear nuclei Obstet Gynecol Papanicolaou smears papillomavirus DNA Pathol pathologists patients polymerase chain reaction primers probes Procedure protein Reagents rescreening risk sample screening sequences sexual slides solution specific specimens squamous intraepithelial lesion staining technique tion Tissue section tumor uterine cervix viral Virol virus women