Cytopathology of the UterusCovers squamous intraepithelial lesions lesions of the endocervix technical considerations etc. |
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Page 34
The basal or germinal cells lie directly on the basal membrane , to which they are
fixed by half desmosomes . These small columnar - shaped cells actively divide
by mitosis and produce all the overlying layers . Next come two or three layers of
...
The basal or germinal cells lie directly on the basal membrane , to which they are
fixed by half desmosomes . These small columnar - shaped cells actively divide
by mitosis and produce all the overlying layers . Next come two or three layers of
...
Page 438
Place each membrane in a plastic bag . 2. Pour 10 mL prehybridization mixture
into the bag . 3. Squeeze out air bubbles and seal . 4. Incubate in water bath at
42 ° C for at least 15 minutes with constant agitation . 20.13E Hybridization
Protocol ...
Place each membrane in a plastic bag . 2. Pour 10 mL prehybridization mixture
into the bag . 3. Squeeze out air bubbles and seal . 4. Incubate in water bath at
42 ° C for at least 15 minutes with constant agitation . 20.13E Hybridization
Protocol ...
Page 439
Procedure 1. Remove membrane from hybridization solution . 2. Wash
membrane with washing solution 1 at room temperature with constant agitation
for a period of 5 minutes . 3. Repeat step 2 . 4. Place the membrane in a new
plastic bag . 5.
Procedure 1. Remove membrane from hybridization solution . 2. Wash
membrane with washing solution 1 at room temperature with constant agitation
for a period of 5 minutes . 3. Repeat step 2 . 4. Place the membrane in a new
plastic bag . 5.
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