Cytopathology of the UterusCovers squamous intraepithelial lesions lesions of the endocervix technical considerations etc. |
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Page 34
The basal or germinal cells lie directly on the basal membrane , to which they are
fixed by half desmosomes . These small columnar - shaped cells actively divide
by mitosis and produce all the overlying layers . Next come two or three layers of
...
The basal or germinal cells lie directly on the basal membrane , to which they are
fixed by half desmosomes . These small columnar - shaped cells actively divide
by mitosis and produce all the overlying layers . Next come two or three layers of
...
Page 438
Place each membrane in a plastic bag . 2. Pour 10 mL prehybridization mixture
into the bag . 3. Squeeze out air bubbles and seal . 4. Incubate in water bath at
42 ° C for at least 15 minutes with constant agitation . 20.13E Hybridization
Protocol ...
Place each membrane in a plastic bag . 2. Pour 10 mL prehybridization mixture
into the bag . 3. Squeeze out air bubbles and seal . 4. Incubate in water bath at
42 ° C for at least 15 minutes with constant agitation . 20.13E Hybridization
Protocol ...
Page 439
Procedure 1. Remove membrane from hybridization solution . 2. Wash
membrane with washing solution 1 at room temperature with constant agitation
for a period of 5 minutes . 3. Repeat step 2 . 4. Place the membrane in a new
plastic bag . 5.
Procedure 1. Remove membrane from hybridization solution . 2. Wash
membrane with washing solution 1 at room temperature with constant agitation
for a period of 5 minutes . 3. Repeat step 2 . 4. Place the membrane in a new
plastic bag . 5.
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Common terms and phrases
abnormal Acta Cytol adenocarcinoma ASCUS associated atypical benign biopsy carcinoma cellular cervical cancer changes chromatin clinical contain cytology cytoplasm demonstrated detection device diagnosis disease dysplasia effect Electron endocervical endometrial enlarged epithelial epithelium et al ethanol evaluation factors Figure gene genital glandular grade HGSIL high-grade hormonal HPV infection HPV types human papillomavirus human papillomavirus infection hybridization Image increased invasive keratinized laboratory layers low power malignant mature medium power membrane metaplasia method minutes morphologic normal nuclear nuclei observed Obstet Gynecol obtained occur Papanicolaou Papanicolaou smears papillo Pathol patients pattern positive Prepared presence probes Procedure production protein reaction risk sample screening seen sequences sexual significance slides smear solution specific specimens squamous cells squamous intraepithelial lesion staining technique tion tissue transformation tumor uterine uterine cervix vaginal viral virus warts women
Bibliographic information
| Title | Cytopathology of the Uterus Volume 1 of ASCP theory and practice of cytopathology |
| Authors | Alexander Meisels, Carol Morin |
| Edition | 2 |
| Publisher | ASCP Press, 1997 |
| Original from | the University of Michigan |
| Digitized | 14 Aug 2008 |
| ISBN | 0891893830, 9780891893837 |
| Length | 506 pages |
| Subjects | › Medical / Gynecology & Obstetrics |
| Export Citation | BiBTeX EndNote RefMan |