Cytopathology of the UterusCovers squamous intraepithelial lesions lesions of the endocervix technical considerations etc. |
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Page 416
Deparaffinize in xylene for 5 minutes . 2. Deparaffinize in xylene for 10 minutes .
3. Dip 10 times in absolute ethanol . 4. Treat with ethanol - formol ( 9 : 1 ) for 5
minutes . 5. Block endogenous peroxidase activity with 3 % H2O , for 30 minutes .
6.
Deparaffinize in xylene for 5 minutes . 2. Deparaffinize in xylene for 10 minutes .
3. Dip 10 times in absolute ethanol . 4. Treat with ethanol - formol ( 9 : 1 ) for 5
minutes . 5. Block endogenous peroxidase activity with 3 % H2O , for 30 minutes .
6.
Page 426
Dehydrate in 50 % ethanol for 15 minutes . 10. Dehydrate in 70 % ethanol for 15
minutes . 11. Dehydrate in 95 % ethanol for 15 minutes . 12. Dehydrate in
absolute ethanol for 15 minutes . 13. Dehydrate in absolute ethanol for 15
minutes . 14.
Dehydrate in 50 % ethanol for 15 minutes . 10. Dehydrate in 70 % ethanol for 15
minutes . 11. Dehydrate in 95 % ethanol for 15 minutes . 12. Dehydrate in
absolute ethanol for 15 minutes . 13. Dehydrate in absolute ethanol for 15
minutes . 14.
Page 434
Deparaffinize tissue sections in xylene for 5 minutes . 5. Deparaffinize tissue ...
Immerse in absolute ethanol for 5 minutes . 8. Immerse in 70 ... Remove excess
enzyme by immersion of slides in PBS for 10 minutes . 13. Postfix tissues in 4 ...
Deparaffinize tissue sections in xylene for 5 minutes . 5. Deparaffinize tissue ...
Immerse in absolute ethanol for 5 minutes . 8. Immerse in 70 ... Remove excess
enzyme by immersion of slides in PBS for 10 minutes . 13. Postfix tissues in 4 ...
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