Cytopathology of the Uterus |
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Page 436
... tube containing 10 mL PBS . c . Swirl the specimen to remove material from the spatula and swab . d . Remove and discard the spatula and swab . e . Centrifuge specimens at 3000 rpm for 20 minutes . f . Remove supernatant fluid from each ...
... tube containing 10 mL PBS . c . Swirl the specimen to remove material from the spatula and swab . d . Remove and discard the spatula and swab . e . Centrifuge specimens at 3000 rpm for 20 minutes . f . Remove supernatant fluid from each ...
Page 447
... tube PBS ( see Procedure 20.12A.1 ) Vortex Tabletop clinical centrifuge 1.5 - mL Eppendorf tubes Low - temperature freezer ( -70 ° C ) or at least a -20 ° C freezer Cell Scrape Specimen Procedure 1. Scrape cervix with a wooden spatula ...
... tube PBS ( see Procedure 20.12A.1 ) Vortex Tabletop clinical centrifuge 1.5 - mL Eppendorf tubes Low - temperature freezer ( -70 ° C ) or at least a -20 ° C freezer Cell Scrape Specimen Procedure 1. Scrape cervix with a wooden spatula ...
Page 449
... tube . 10. Discard organic phase . 11. Add 5 mL chloroform - isoamyl alcohol ( 24 : 1 ) to the tube containing DNA . 12. Mix slowly by inversion for 5 minutes . 13. Centrifuge at 6000 rpm for 5 minutes . 14. Remove upper aqueous layer ...
... tube . 10. Discard organic phase . 11. Add 5 mL chloroform - isoamyl alcohol ( 24 : 1 ) to the tube containing DNA . 12. Mix slowly by inversion for 5 minutes . 13. Centrifuge at 6000 rpm for 5 minutes . 14. Remove upper aqueous layer ...
Contents
Hormonal Cytology | 6 |
J Ernest Ayre | 12 |
The Procurement of Cytologic Specimens | 19 |
Copyright | |
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abnormal acid Acta Cytol adenocarcinoma antibody antigen ASCUS atypia benign Bethesda System biopsy cell carcinoma cellular cervical cancer cervical carcinoma cervical intraepithelial neoplasia cervical smears chromatin Clin clinical colposcopic condyloma contain cytology cytopathology cytoplasm cytotechnologists detection diagnosis distilled water double-distilled water dysplasia Electron endocervical endometrial cancer endometrium Epidemiology epithelial estrogen ethanol Figure gene genital warts genome glandular cells HGSIL high-grade squamous intraepithelial histologic hormonal HPV infection HPV types HPV-DNA human papillo human papillomavirus human papillomavirus infection human papillomavirus type hybridization Image invasive keratin koilocytes laboratory low power malignant mavirus medium power Meisels membrane microscope minutes mol/L morphologic nuclear nuclei Obstet Gynecol Papanicolaou smears papillomavirus DNA Pathol pathologists patients polymerase chain reaction primers probes Procedure protein Reagents rescreening risk sample screening sequences sexual slides solution specific specimens squamous intraepithelial lesion staining technique tion Tissue section tumor uterine cervix viral Virol virus women