PCR Methods and Applications, Volume 1

Acad agarose gel allele-specific alleles amplifica amplification products amplified DNA analysis annealing assay bands base Biol buffer cDNA cells Cetus Corporation chromosome clones codon Cold Spring Harbor coli containing contig cycles denaturation detection digestion direct sequencing DNA fragments DNA polymerase DNA sequence dNTP enzymatic fidelity FIGURE gel electrophoresis genetic genomic genomic DNA H.A. Erlich human hybridization incubated influenza isolated labeled lane ligase ligation mapping markers ment method MgCl2 molecular molecules mRNA Natl Nucleic Acids Nucleic Acids Res nucleotide oligonucleotide PCR amplification PCR primers PCR products PCR reaction plasmid polymerase chain reaction polymorphism probes Proc procedure protein protocol pseudogene quantitative quence ratio reac region restriction enzyme reverse transcriptase rRNA samples screening Sequenase single-stranded specific strand STSS synthesis Taq polymerase target temperature template thermal cycler thermostable tion tissue transcription transgenic vector Vent virus vitro yeast artificial chromosomes