Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-42
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
Page 8-74
... Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged into infectious bacteriophage particles , it is essential to use packaging ...
... Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged into infectious bacteriophage particles , it is essential to use packaging ...
Page 15-72
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml