Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-3
... DNA with restriction enzymes . These DNA fragments were fractionated by density gradient centrifugation or prepara- tive gel electrophoresis to select those whose size was suitable for insertion into the vector ( Maniatis et al . 1978 ) ...
... DNA with restriction enzymes . These DNA fragments were fractionated by density gradient centrifugation or prepara- tive gel electrophoresis to select those whose size was suitable for insertion into the vector ( Maniatis et al . 1978 ) ...
Page 15-48
... fragments whose lengths are accurately known and whose sizes are approximately equal to that of the target DNA . Such markers can be easily generated by cleaving the original recombi- nant plasmid ... DNA EXCISION OF THE TARGET DNA 15 48.
... fragments whose lengths are accurately known and whose sizes are approximately equal to that of the target DNA . Such markers can be easily generated by cleaving the original recombi- nant plasmid ... DNA EXCISION OF THE TARGET DNA 15 48.
Page 15-92
... DNA fragment carrying the kan ' gene has been inserted into the amp ' gene rather than into the target sequence . 9. Pick 36 independent transformants , and establish small - scale ( 2 - ml ) cultures in LB medium containing ampicillin ...
... DNA fragment carrying the kan ' gene has been inserted into the amp ' gene rather than into the target sequence . 9. Pick 36 independent transformants , and establish small - scale ( 2 - ml ) cultures in LB medium containing ampicillin ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml