Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-18
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the ... DNA . 13.18 DNA Sequencing.
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the ... DNA . 13.18 DNA Sequencing.
Page 13-102
... DNA sequencing : Chemical methods . Methods Enzymol . 152 : 522 . Anderson , S. , A.T. Bankier , B.G. Barrell , M.H.L. de Bruijn , A.R. Coulson , J. Drouin , I.C. Eperon ... DNA sequenc- 13.102 DNA Sequencing References 13 L L L 105 2.
... DNA sequencing : Chemical methods . Methods Enzymol . 152 : 522 . Anderson , S. , A.T. Bankier , B.G. Barrell , M.H.L. de Bruijn , A.R. Coulson , J. Drouin , I.C. Eperon ... DNA sequenc- 13.102 DNA Sequencing References 13 L L L 105 2.
Page 13-103
... G.F. Hong , D.F. Hill , and G.B. Petersen . 1982. Nu- cleotide sequence of bacteriophage A DNA . J. Mol . Biol . 162 : 729 . Sanger , F. , J.E. Donelson , A.R. Coulson , H. Kössel , and D. Fischer . 1973. Use of DNA Sequencing 13.103.
... G.F. Hong , D.F. Hill , and G.B. Petersen . 1982. Nu- cleotide sequence of bacteriophage A DNA . J. Mol . Biol . 162 : 729 . Sanger , F. , J.E. Donelson , A.R. Coulson , H. Kössel , and D. Fischer . 1973. Use of DNA Sequencing 13.103.
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml