Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-9
... DNAase I added to the reaction and the amount of DNAase contaminating the preparation of E. coli DNA polymerase I also affect the size of the DNA after nick translation . Because the amount of contaminating nuclease differs widely ...
... DNAase I added to the reaction and the amount of DNAase contaminating the preparation of E. coli DNA polymerase I also affect the size of the DNA after nick translation . Because the amount of contaminating nuclease differs widely ...
Page 10-11
... DNAase I to generate nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower efficiencies of label incorporation . It therefore seems that DNAase I is rate - limiting in the reaction and ...
... DNAase I to generate nicks in the template . Interestingly , the lag is longer when using preparations of DNA that give lower efficiencies of label incorporation . It therefore seems that DNAase I is rate - limiting in the reaction and ...
Page 15-27
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml