Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 52
Page 8-59
... ( pH 7.6 and pH 8.0 ) Trichloroacetic acid ( TCA ) ( 10 % ) Tris base ( 2 M ) Tris Cl ( 2 м ; pH 7.4 ) · Tris Cl ( 1 м ... EDTA ( pH 8.0 ) . This reagent is used to buffer the synthesis of the first strand of cDNA catalyzed by reverse ...
... ( pH 7.6 and pH 8.0 ) Trichloroacetic acid ( TCA ) ( 10 % ) Tris base ( 2 M ) Tris Cl ( 2 м ; pH 7.4 ) · Tris Cl ( 1 м ... EDTA ( pH 8.0 ) . This reagent is used to buffer the synthesis of the first strand of cDNA catalyzed by reverse ...
Page 9-16
... ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS [ It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the ...
... ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS [ It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the ...
Page 9-20
... ( pH 8.0 ) , 10 mм EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris · Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . . 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
... ( pH 8.0 ) , 10 mм EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris · Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . . 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml