Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-82
... G.P. , K. Horiuchi , and N.D. Zinder . 1984. The functional origin of ... C.P. Hodgson , H.L. Moses , and M.J. Getz . 1982. Poly- adenylated RNA ... G.P. Thomas , and S.H. Hughes . 1983. 8.82 Construction and Analysis of cDNA Libraries.
... G.P. , K. Horiuchi , and N.D. Zinder . 1984. The functional origin of ... C.P. Hodgson , H.L. Moses , and M.J. Getz . 1982. Poly- adenylated RNA ... G.P. Thomas , and S.H. Hughes . 1983. 8.82 Construction and Analysis of cDNA Libraries.
Page 10-51
... Cp Cp Tp Ap Gp 5 [ a - 32P ] dGTP G OH 3 ' Со сот G A G & G OH 3 ' 5 ' Blunt - ended DNA fragments are labeled by replacement of the nucleotide present at the 3 ' - hydroxyl terminus . C 5 p [ a - 32P ] dGTP T 5 ' • AG OH 3 ' A DG OH 3 ...
... Cp Cp Tp Ap Gp 5 [ a - 32P ] dGTP G OH 3 ' Со сот G A G & G OH 3 ' 5 ' Blunt - ended DNA fragments are labeled by replacement of the nucleotide present at the 3 ' - hydroxyl terminus . C 5 p [ a - 32P ] dGTP T 5 ' • AG OH 3 ' A DG OH 3 ...
Page 10-53
... Gp 5 ' [ a - 32P ] dGTP 5 ' 5 ̊ PA PA PT T PC G 3 ' A ** G OH 3 ' Ap Gp 5 5 ' pA pA pT pT pC рос 3 ' HO G GG OH 3 ' Cp Cp Tp Ap Gp 5 ' LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA Preparation of Radiolabeled DNA and RNA Probes 10.53.
... Gp 5 ' [ a - 32P ] dGTP 5 ' 5 ̊ PA PA PT T PC G 3 ' A ** G OH 3 ' Ap Gp 5 5 ' pA pA pT pT pC рос 3 ' HO G GG OH 3 ' Cp Cp Tp Ap Gp 5 ' LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA Preparation of Radiolabeled DNA and RNA Probes 10.53.
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml