Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-40
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible ... KLENOW FRAGMENT OF E coli DNA POLYMERASE I 11 40.
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible ... KLENOW FRAGMENT OF E coli DNA POLYMERASE I 11 40.
Page 13-79
... Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed terminus with the appropriate [ a - 32P ] dNTP and ...
... Klenow fragment of E. coli DNA polymerase I 32 PN Isolate target DNA by cleavage with two restriction enzymes , only one of which generates a recessed 3 ' terminus Radiolabel recessed terminus with the appropriate [ a - 32P ] dNTP and ...
Page 14-7
... Klenow fragment of E. coli DNA polymerase I and analyzed the sequences of seven independent clones that contained an amplified DNA fragment of porcine urate oxidase cDNA 112 bp in length . Although the sequences of all seven clones of ...
... Klenow fragment of E. coli DNA polymerase I and analyzed the sequences of seven independent clones that contained an amplified DNA fragment of porcine urate oxidase cDNA 112 bp in length . Although the sequences of all seven clones of ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml