Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xix
... SUBSTRATES 10.60 USING DNA MOLECULES WITH BLUNT ENDS OR RECESSED 5 ' TERMINI AS SUBSTRATES 10.62 DEPHOSPHORYLATION OF DNA 10.64 Exchange Reaction 10.66 USING DNA MOLECULES WITH PROTRUDING 5 ' - PHOSPHATE TERMINI AS TEMPLATES 10.66 ...
... SUBSTRATES 10.60 USING DNA MOLECULES WITH BLUNT ENDS OR RECESSED 5 ' TERMINI AS SUBSTRATES 10.62 DEPHOSPHORYLATION OF DNA 10.64 Exchange Reaction 10.66 USING DNA MOLECULES WITH PROTRUDING 5 ' - PHOSPHATE TERMINI AS TEMPLATES 10.66 ...
Page 10-60
... SUBSTRATES 1. Mix : 5 ' termini of dephosphorylated DNA ( see pages 10.64-10.65 ) 10 bacteriophage T4 polynucleotide kinase buffer [ y - 32P ] ATP ( sp . act . 3000 Ci / mmole ; 10 Ci / ml ) bacteriophage T4 polynucleotide kinase H2O to ...
... SUBSTRATES 1. Mix : 5 ' termini of dephosphorylated DNA ( see pages 10.64-10.65 ) 10 bacteriophage T4 polynucleotide kinase buffer [ y - 32P ] ATP ( sp . act . 3000 Ci / mmole ; 10 Ci / ml ) bacteriophage T4 polynucleotide kinase H2O to ...
Page 12-19
... substrate such as 4 - chloro - 1 - naphthol . To prepare de- veloping solution , dissolve 60 mg of this substrate in 20 ml of ice - cold methanol . Just before use , mix the solution with 100 ml of 10 mм Tris . Cl ( pH 7.5 ) , 150 mM ...
... substrate such as 4 - chloro - 1 - naphthol . To prepare de- veloping solution , dissolve 60 mg of this substrate in 20 ml of ice - cold methanol . Just before use , mix the solution with 100 ml of 10 mм Tris . Cl ( pH 7.5 ) , 150 mM ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml