Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-16
... acetate electrophoresis buffer ( see Chapter 6 ) ( Feinberg and Vogelstein 1984 ) . 1. After electrophoresis ( in Tris - acetate electrophoresis buffer ) , stain the gel with ethidium bromide ( 0.5 μg / ml in water ) , and excise the ...
... acetate electrophoresis buffer ( see Chapter 6 ) ( Feinberg and Vogelstein 1984 ) . 1. After electrophoresis ( in Tris - acetate electrophoresis buffer ) , stain the gel with ethidium bromide ( 0.5 μg / ml in water ) , and excise the ...
Page 10-54
... acetate ( pH 8.0 ) 0.66 м potassium acetate 0.1 M magnesium acetate 5 mm dithiothreitol 0.1-2 με 2 μl 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 stock should be divided into small aliquots and stored frozen at -20 ° C ...
... acetate ( pH 8.0 ) 0.66 м potassium acetate 0.1 M magnesium acetate 5 mm dithiothreitol 0.1-2 με 2 μl 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) The 10 stock should be divided into small aliquots and stored frozen at -20 ° C ...
Page 13-98
... acetate ) , after which piperidine breaks the DNA at guanines . Residual sodium acetate from the second ethanol precipitation buffers piperidine , leading to incomplete phosphate elimina- tion reactions , which require a high pH . The 3 ...
... acetate ) , after which piperidine breaks the DNA at guanines . Residual sodium acetate from the second ethanol precipitation buffers piperidine , leading to incomplete phosphate elimina- tion reactions , which require a high pH . The 3 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml