Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-23
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
... Acrylamide stock acrylamide ( DNA - sequencing grade ) N , N ' - methylenebisacrylamide H2O to 250 ml 190 g 10 g Dissolve the chemicals by heating the solution to 37 ° C . Adjust the volume of the solution to 500 ml . Filter the ...
Page 11-43
... acrylamide gel ( i.e. , cast with 7 M urea ) . Gel - loading buffer 80 % w / v formamide 50 mM Tris - borate ( pH ... Acrylamide is a potent neurotoxin and is absorbed through the skin . The effects of acrylamide are cumulative . Wear ...
... acrylamide gel ( i.e. , cast with 7 M urea ) . Gel - loading buffer 80 % w / v formamide 50 mM Tris - borate ( pH ... Acrylamide is a potent neurotoxin and is absorbed through the skin . The effects of acrylamide are cumulative . Wear ...
Page 13-48
... Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
... Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml