Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 15
Page 12-23
... agar plate to a nitrocellulose filter . The method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results : They produce sharper signals and smear less than larger colonies . As many as 2 ...
... agar plate to a nitrocellulose filter . The method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results : They produce sharper signals and smear less than larger colonies . As many as 2 ...
Page 15-65
... agar ( melted and cooled to 45 ° C ) to each culture , and plate each mixture on a separate YT agar plate . Incubate the plates for 16 hours at 37 ° C to allow plaques to form . If mutagenesis is carried out by the Kunkel method ( see ...
... agar ( melted and cooled to 45 ° C ) to each culture , and plate each mixture on a separate YT agar plate . Incubate the plates for 16 hours at 37 ° C to allow plaques to form . If mutagenesis is carried out by the Kunkel method ( see ...
Page 15-70
... plate the entire mixture on a single YT agar plate . Incubate the plate for 16 hours at 37 ° C to allow plaques to form . e . Rescreen the plaques with the radiolabeled oligonucleotide as de- scribed in steps 1-9 . In this second round ...
... plate the entire mixture on a single YT agar plate . Incubate the plate for 16 hours at 37 ° C to allow plaques to form . e . Rescreen the plaques with the radiolabeled oligonucleotide as de- scribed in steps 1-9 . In this second round ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml