Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 ...
Page 9-34
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS There are three methods to transfer fragments of DNA from agarose gels to solid supports ( nitrocellulose filters or nylon membranes ) ...
Page 9-41
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
... AGAROSE GEL TO TWO NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml