Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-49
... amino acid sequence of the protein of interest . Because of the degeneracy of the genetic code , it is very unlikely that a given sequence of amino acids will be specified by a predictable single oligonucleotide of defined sequence ...
... amino acid sequence of the protein of interest . Because of the degeneracy of the genetic code , it is very unlikely that a given sequence of amino acids will be specified by a predictable single oligonucleotide of defined sequence ...
Page 11-9
... amino acids that are specified by only one or two codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure ...
... amino acids that are specified by only one or two codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure ...
Page 15-83
... amino acid sequences . Residues that are highly conserved between proteins of different function are more likely to be involved in forming common structural motifs than in highly specific and private activities such as catalysis or ...
... amino acid sequences . Residues that are highly conserved between proteins of different function are more likely to be involved in forming common structural motifs than in highly specific and private activities such as catalysis or ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml