Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 9
Page 12-38
... ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 ...
... ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 . Transfer four 100 - μl aliquots of the diluted culture to fresh culture tubes . 4. To three of the tubes , add 1 ...
Page 15-44
... ampicillin ( i.e. , the bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C until step 2 of the ...
... ampicillin ( i.e. , the bacterial plates used to select transformants should contain both kanamycin and ampicillin , each at a concentration of 100 μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C until step 2 of the ...
Page 15-92
... ampicillin and kanamycin , each at a concentration of 100 μg / ml . After the cultures have reached saturation , isolate the plasmid DNA using one of the methods described in Chapter 1. Analyze the DNAs by digestion with the appropriate ...
... ampicillin and kanamycin , each at a concentration of 100 μg / ml . After the cultures have reached saturation , isolate the plasmid DNA using one of the methods described in Chapter 1. Analyze the DNAs by digestion with the appropriate ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml