Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 14-5
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
Page 14-10
... amplification , one primer is essentially depleted and the final stages of the amplification reaction therefore generate copies of only one strand of the original DNA ( Gyllen- sten and Erlich 1988 ; Mihovilovic and Lee 1989 ) ...
... amplification , one primer is essentially depleted and the final stages of the amplification reaction therefore generate copies of only one strand of the original DNA ( Gyllen- sten and Erlich 1988 ; Mihovilovic and Lee 1989 ) ...
Page 14-28
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml