Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 54
Page 8-50
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
... antibody . It is essential that the antibody efficiently recognize the denatured protein ( i.e. , it should produce strong signals on western blots ) . Screening is made easier and more sensitive if , in addition , the antibody is ...
Page 12-19
... antibody diluted in blocking buffer ( 7.5 ml for each 82 - mm filter ; 15 ml for each 138 - mm filter ) . Use the highest dilution of antibody that gives acceptable background yet still allows detection of 50-100 pg of denatured antigen ...
... antibody diluted in blocking buffer ( 7.5 ml for each 82 - mm filter ; 15 ml for each 138 - mm filter ) . Use the highest dilution of antibody that gives acceptable background yet still allows detection of 50-100 pg of denatured antigen ...
Page 12-20
... antibody - antibody - AP complexes are located using the sub- strate 5 - bromo - 4 - chloro - 3 - indolyl phosphate ( BCIP ) in combination with nitro blue tetrazolium ( NBT ) , which detects the precipitated indoxyl group . Stock ...
... antibody - antibody - AP complexes are located using the sub- strate 5 - bromo - 4 - chloro - 3 - indolyl phosphate ( BCIP ) in combination with nitro blue tetrazolium ( NBT ) , which detects the precipitated indoxyl group . Stock ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml