Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-18
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
Page 9-47
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
Page 15-42
... aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ethanol to the aqueous phase , and ...
... aqueous phases by centrifugation at 12,000g for 3 minutes at 4 ° C in a microfuge , and transfer the aqueous phase to a fresh microfuge tube . Add 0.5 volume of 5 м ammonium acetate and 2.5 volumes of ethanol to the aqueous phase , and ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml