Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxx
... Bacterial Strains LIQUID MEDIA A.1 LB Medium ( Luria - Bertani medium ) A.1 NZCYM Medium A.1 NZYM Medium NZM Medium ... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 ...
... Bacterial Strains LIQUID MEDIA A.1 LB Medium ( Luria - Bertani medium ) A.1 NZCYM Medium A.1 NZYM Medium NZM Medium ... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 ...
Page 15-72
... bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the plates at 4 ° C until they are needed . · Lyse the colonies transferred to the filter with alkali ( see Chapter 1 ) . Neutralize the filters ...
... bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the plates at 4 ° C until they are needed . · Lyse the colonies transferred to the filter with alkali ( see Chapter 1 ) . Neutralize the filters ...
Page 15-76
... bacterial cells . Vortex the tube vigorously for 30 seconds to release the bacteriophages trapped in the top agar , and then centrifuge the tube at 12,000g for 2 minutes at 4 ° C in a microfuge . This eliminates dead bacterial cells and ...
... bacterial cells . Vortex the tube vigorously for 30 seconds to release the bacteriophages trapped in the top agar , and then centrifuge the tube at 12,000g for 2 minutes at 4 ° C in a microfuge . This eliminates dead bacterial cells and ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml