Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xi
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
Page 15-63
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
Page 15-74
... M13 bacteriophages grown in such strains contain between 20 and 30 uracil residues in their DNA . When these ... bacteriophage M13 recombinant in a strain of E. coli that is dut ung F ' . The resulting uracil - containing single ...
... M13 bacteriophages grown in such strains contain between 20 and 30 uracil residues in their DNA . When these ... bacteriophage M13 recombinant in a strain of E. coli that is dut ung F ' . The resulting uracil - containing single ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml