Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-56
... Bacteriophage λ arms Bacteriophage T4 DNA ligase Required only if self - priming of second - strand cDNA synthesis in un- acceptably high ( see note to step 5g on page 8.65 ) . Preparations of actinomycin D supplied by pharmaceutical ...
... Bacteriophage λ arms Bacteriophage T4 DNA ligase Required only if self - priming of second - strand cDNA synthesis in un- acceptably high ( see note to step 5g on page 8.65 ) . Preparations of actinomycin D supplied by pharmaceutical ...
Page 10-54
... BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , bacteriophage T4 ...
... BACTERIOPHAGE T4 DNA POLYMERASE Rapid End - labeling of DNA Bacteriophage T4 DNA polymerase , like the Klenow fragment of E. coli DNA polymerase I , can be used to label flush or recessed 3 ' termini of DNA . However , bacteriophage T4 ...
Page 11-31
... BACTERIOPHAGE T4 POLYNUCLEOTIDE KINASE Synthetic oligonucleotides are synthesized without a phosphate group at their 5 ' termini and are therefore easily labeled by transfer of the y - 32P from [ y - 32P ] ATP using the enzyme bacteriophage ...
... BACTERIOPHAGE T4 POLYNUCLEOTIDE KINASE Synthetic oligonucleotides are synthesized without a phosphate group at their 5 ' termini and are therefore easily labeled by transfer of the y - 32P from [ y - 32P ] ATP using the enzyme bacteriophage ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml