Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 11
Page 11-27
... bromophenol blue ) . 11. Run the gel at 1500 V until the oligonucleotide has migrated approxi- mately two thirds of the length of the gel . If necessary , a metal plate can be clamped to the gel to help eliminate curvature of the bands ...
... bromophenol blue ) . 11. Run the gel at 1500 V until the oligonucleotide has migrated approxi- mately two thirds of the length of the gel . If necessary , a metal plate can be clamped to the gel to help eliminate curvature of the bands ...
Page 13-48
... bromophenol blue 10 mg Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45- micron pore size ) . The 6 % acrylamide / urea bottom solution may be stored for several weeks at 4 ° C . sev The sucrose is added to ...
... bromophenol blue 10 mg Filter the solution through a nitrocellulose filter ( e.g. , Nalge , 0.45- micron pore size ) . The 6 % acrylamide / urea bottom solution may be stored for several weeks at 4 ° C . sev The sucrose is added to ...
Page 13-55
... bromophenol blue from the first loading buffer has migrated from the gradient gel ( ~ 3 hours ) . The sequence obtained from the first loading will be more distal to the primer , whereas that obtained from the second loading will be ...
... bromophenol blue from the first loading buffer has migrated from the gradient gel ( ~ 3 hours ) . The sequence obtained from the first loading will be more distal to the primer , whereas that obtained from the second loading will be ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml