Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-47
... carried out under stringent conditions . Partially homologous probes Partially homologous probes are used to detect cDNA clones that are related , but not identical , to the probe sequences . If neither antibody nor nucleic acid probes ...
... carried out under stringent conditions . Partially homologous probes Partially homologous probes are used to detect cDNA clones that are related , but not identical , to the probe sequences . If neither antibody nor nucleic acid probes ...
Page 11-45
... carried out at 15-25 ° C below the calculated melting temperature ( T ) of a perfect hybrid . However , as the length of the probe is decreased , the Tm is lowered to the point where it is often impractical to carry out hybridization at ...
... carried out at 15-25 ° C below the calculated melting temperature ( T ) of a perfect hybrid . However , as the length of the probe is decreased , the Tm is lowered to the point where it is often impractical to carry out hybridization at ...
Page 13-43
... carried out synchronously . Some laboratories carry out all stages of sequencing reactions - from annealing to chain termination - in microtiter plates . In our experience , this is not advisable . During annealing , when small volumes ...
... carried out synchronously . Some laboratories carry out all stages of sequencing reactions - from annealing to chain termination - in microtiter plates . In our experience , this is not advisable . During annealing , when small volumes ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml