Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-37
... carry an intact cI gene is therefore suppressed on strains of E. coli that carry an hfl mutation . In contrast , the growth of recombinant bacteriophages that cannot synthesize an active cI gene product is not inhibited ( see Hoyt et al ...
... carry an intact cI gene is therefore suppressed on strains of E. coli that carry an hfl mutation . In contrast , the growth of recombinant bacteriophages that cannot synthesize an active cI gene product is not inhibited ( see Hoyt et al ...
Page 8-41
... carry the sequence pTT , at their 5 ' termini will generate unmethylated HindIII sites at both their 5 ' and 3 ' termini . Neither class of cDNA can be cloned into AORF8 by the directional method . Finally , in comparison to Agt11 ...
... carry the sequence pTT , at their 5 ' termini will generate unmethylated HindIII sites at both their 5 ' and 3 ' termini . Neither class of cDNA can be cloned into AORF8 by the directional method . Finally , in comparison to Agt11 ...
Page 15-44
... carry a kan gene and therefore a BglII linker in the target region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) yielded ...
... carry a kan gene and therefore a BglII linker in the target region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) yielded ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml