Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 40
Page 10-35
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 8. Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the bench for a few minutes to allow the last visible traces of ethanol to ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 8. Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the bench for a few minutes to allow the last visible traces of ethanol to ...
Page 13-72
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 7. Carefully remove the supernatants by aspiration , and add 1 ml of 70 % ethanol to each tube . Vortex the tube briefly , and then recentrifuge at 12,000g for 5 minutes ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . 7. Carefully remove the supernatants by aspiration , and add 1 ml of 70 % ethanol to each tube . Vortex the tube briefly , and then recentrifuge at 12,000g for 5 minutes ...
Page 15-42
... centrifugation at 12,000g for 15 minutes at 4 ° C in a microfuge . Carefully decant the ethanol , and wash the pellet of DNA with 300 μl of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 ...
... centrifugation at 12,000g for 15 minutes at 4 ° C in a microfuge . Carefully decant the ethanol , and wash the pellet of DNA with 300 μl of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
Copyright | |
82 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml