Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-9
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
... codons . Regions rich in amino acids with six codon choices ( Leu , Ser , and Arg ) should be avoided . The number of codons that encode each amino acid are given below ( see also Figure 11.1 ) . 1 2 3 4 6 Met Phe Ile Val Leu Trp Tyr ...
Page 11-11
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
... codons that differ only in the third position , so that at least two of the three nucleotides of each codon are guaranteed to be perfectly matched . Hybridization can therefore be carried out under comparatively stringent conditions ...
Page 11-14
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
... codon choices that generate the sequence CpG between codons . This dinucleotide is significantly underrepresented in mammalian DNA ( Bird 1980 ) , and CpG occurs in intercodons at about one half of the expected frequency ( Lathe 1985 ) ...
Contents
E | 8-8 |
SYNTHESIS OF THE FIRST STRAND OF CDNA 8 | 8-11 |
CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH BUTANOL E | 8-16 |
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acrylamide agarose gel aliquots amplification annealing antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g cleavage cleaved cleotide coli DNA polymerase concentration containing ddNTPs deletions denatured digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers Maxam-Gilbert method MgCl2 microfuge tube minutes at 4°C molecules mRNA Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid piperidine plaques plasmid plasmid DNA plate polymerase chain reaction precipitation prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing gel sequencing reactions sonicated specific activity stored strand of cDNA synthesis T4 DNA polymerase target DNA target sequence template DNA termini transcription transfer Tris Cl pH vectors µg/ml